HISTOMORPHOMETRIC INVESTIGATIONS OF SPONTANEOUS CANINE MAMMARY GLAND TUMOURS
DOI:
https://doi.org/10.15547/tjs.2025.s.01.001Keywords:
canine mammary gland tumours, quantitative morphologyAbstract
Histomorphometric analysis was performed on preparations of 18 spontaneous canine mammary epithelial neoplasias (fibroadenoma (n=6), tubulopapillary carcinoma (n=6) and solid carcinoma (n=6). Computer histomorphometric analysis of cell nuclei was performed using a digital microscope and morphometric analysis software (Image Pro PlusÒ v.4.5 Media Cybernetics, Silver Spring, MD, USA). The studied morphometric parameters were mean nuclear area (MNA, μm2), mean nuclear perimeter (MNP, μm) and mean nuclear diameter (D mean, μm). The analysis of our results shows that there are reliable statistical differences between benign and malignant mammary neoplasms. In this regard, histomorphometric analysis can be used to differentiate benign from malignant mammary neoplasias in the bitch. On the other hand, however, statistical analysis shows that this study does not allow differentiation between malignant mammary neoplasms.
References
Dorn, C., Taylor D. and Schneider, R., Survey of animal neoplasms in Alameda and Contra Costa Counties, California. II. Cancer morbidity in dogs and cats from Alameda Country. J Natl Cancer Instite, 40: 307-318, 1968.
Bostock, D., Canine and feline mammary neoplasms. Br Vet J, 142: 506-515, 1986.
Moe, L., Population-based incidence of mammary tumors in some dog breeds. J Reprod Fertil, 57: 439-443, 2001.
Morris, J and Dobson, J., Mammary gland. In: Morris J. Dobson J (eds), Small Animal Oncology Iowa State University Press, 184-192, 2001.
Misdorp, B., Else R., Hellmen E. and Lipscomb T., Histological classification of mammary tumors of the dog and cat. WHO International Histological Classification of Tumors in Domestic Animals. 2nd series, Vol VII. Washington DC: Armed Forces Institute of Pathology, American Registry of Pathology, 2001.
Sorenmo, K., Canine mammary gland tumors. Vet Clin Small Anim, 33: 573-596, 2003.
Brodey, R., Goldschmidt M. and Roszel, J., Canine mammary gland neoplasms. J Am Anim Hosp Assoc, 19: 61-90, 1983.
Yager, J., Scott, D. and Misdorp, W., The skin and appedages. In: Jubb K, Kennedi P. and Palmer, N (eds). Pathology of Domestic Animals. ed 4. Academic Press, San Diego, 1993.
Zhelev, V., Dzhurov, A. and Angelov, A., Studies on the spread and localization of tumors in domestic animals and birds. Scientific works of the Institute of Veterinary Medicine, volume XVI, 1966.
Tsvetkov, Y. Pathomorphological studies and some epizootological features of tumors in dogs. Dissertation, Sofia, 1998
Dinev, I., Dimov D., Parvanov P., Georgiev P. and Simeonova, G., Incidence of canine neoplasms-a retrospective histopathological study. I. Mammary neoplasms in the bitch. BJVM, 5(3): 195-204, 2002.
Allen, S., Prasse K. and Mahaffey, E., Cytologic differentiation of benign from malignant canine mammary tumors. Vet Pathol, 23: 649-655, 1986.
Allison, M., Bain P. and Latimer, K., Canine mammary carcinoma. http://www.vet.uga.edu/vpp/clerk/mccarthy/, 2003.
Simeonov. R., Cytological and computer cytomorphological studies in spontaneous mammary neoplasias in bitches. Dissertation, 2006.
Ciurea, D., Wilkins R., Shalev M., Liu Z., Barba B. and Gil, J., Use of computerezed interactive morphometry in the diagnosis of mammary adenoma and adenocarcinoma in dogs. Am J Vet Res, 53: 300-303, 1992
Juntes, P. and Pogacnik, M., Morphometric analysis of AgNOR in tubular and papillary parts of canine mammary gland tumors. Anal Quant Cytol Histol, 22: 185-192, 2000.
Dobreva, A., Cytological diagnostics of pre-cancerous diseases and mammary gland cancer. Dissertation, Sofia, 1980.
Denchev, D., Quantitative morphology and diagnostics of pre-tumor and tumor processes in the mammary gland. Dissertation. Sofia, 1988.
Dey, P., Ghoshal S. and Pattari, S., Nuclear image morphometry and cytologic grade of breast carcinoma. Anal Quant Cytol Histol, 22: 483-485, 2000.
Tahlan, A., Nijhawan R. and Joshi, J., Grading of ductal breast carcinoma by cytomorphology and image morphometry with histologic correlation. Anal Quant Cytol Histol, 22: 193-198, 2000.
Tan, P., Goh B., Chiang G. and Bay, B., Correlation of nuclear morphometry with pathologic parameters in ductal carcinoma in situ in the breast. Mod Pathol, 14: 937-941, 2001.
Rajesh, L., Dey P. and Joshi, K., Automated image morphometry of lobular breast carcinoma. Anal Quant Cytol Histol, 24: 81-84, 2002.
Fiorella, R., Kragel P., Shariff A. and Dubey, S., Fine-needle aspiration of well differentiated small-cell duct carcinoma of the breast. Diagn Cytopathol, 16: 226-229, 1997.
Ladecarl, M., The influence of tissue processing on quantitative histopathology in breast cancer. J Microscop, 174: 93-100, 1994.
De Vico, G., Sfacteria A., Maiolino P. and Mazzullo, G., Comparison of nuclear morpfometric parameters in cytologic smears and histologic section of spontaneous canine tumors. Vet Clin Pathol, 31: 16-18, 2002.
Elzagheid, A. and Collan, Y,. Fine-needle aspiration biopsy of the breast. Value of nuclear morphometry after different sampling methods. Anal Quan Cytol Histol, 25: 43-80, 2003
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
